Light Microscopy at the Nano Research Facility

Leica SUPER RESOLUTION STED MICROSCOPe

The Leica TCS SP8 STED super-resolution microscope is based on the STED (STimulated Emission Depletion) principle developed by Nobel Prize laureate Stefan Hell.  The base of this system is Fluorescent Confocal Microscope which suitable for use in top level biological and biomedical research and surface analysis in material science applications.

• The light sources include a pulsed 405 nm diode laser, a white light Laser which can provide excitation wavelengths from 470 nm - 670 nm, and a metal halide lamp for wide field imaging

• 592 nm and 660 nm STED depletion lasers for super resolution imaging.

• Fluorescent Lifetime Microscopy using HyD single molecule detectors

• FLIM, FCS, FLCS, FRAP and FRET measurements.

• 2 Internal standard PMT confocal detectors, 2 Internal HyD GaAsP SMD detectors for gated imaging and 1 CCD camera

• 10x air, 20x, 40x and 63x (with motorized correction collar) water, 40x, 63x and 100x oil objectives.

• Resonant scanner for imaging at 24 frames per second.

• Galvoflow, SuperZ galvanometer

• 37°C degree stage top CO² incubator for live cell imaging

• Has both high resolution and flexibility to cover a wide range of experimental setups in fluorescent microscopy

Keyence 3D Digital Microscope

• Large Depth of Field

• Large magnification range (0.1 x – 5000 x)

• 360 degree observation

• 2D / 3D imaging and measurement capabilities

• Motorised and automated XYZ

• High speed image stitching

• High Dynamic Range (HDR)

• Depth composition function for full focus imaging

• 54 megapixel 3CCD camera