Spectroscopy at the Nano Research Facility

Spectroscopy

Circular Dichroism Spectrometer

Applied Photophysics Chirascan Plus Circular Dichroism Spectrometer

Applied Photophysics Chirascan Plus

Provides researchers with structural information of biomolecules and synthetic biomimetic polymers. 

Capable of simultaneous data acquisition of parameters such as: Circular Dichroism, Fluorescence, true UV-VIS Absorbance, Temperature and Single/Multi-Wavelength Thermal Melt Analysis, across a wide wavelength range (170-1,150nm). Using the same high-performance UV-VIS-IR avalanche photo-diode detector, it is also possible to configure the instrument for the acquisition of both Linear Dichroism measurements (down to 180nm) and Optical Rotatory Dispersion measurements. The sample compartment contains a single cell Peltier-controlled cell holder which is capable of performing temperature-controlled (ramping) measurements with the ability to acquire Circular Dichroism vs temperature curves at more than one wavelength in a single continuous-temperature-ramping experiment (with the sample temperature recorded for each discrete measurement). 

  • Wavelength accuracy of ±0.2nm between 170-400nm & ±0.5nm above 400nm
  • Wavelength precision of ±0.05nm between 170-400nm & ±0.1nm above 400nm
  • Resolution of ±0.1nm
  • Stray light levels below 5ppm @ 200nm and below 8ppm @ 180nm
  • Baseline stability of ±0.02mdeg/hr (@ 290nm)
  • RMS noise levels between 0.02mdeg - 0.05mdeg (depending on wavelength)

Prices:

Academic:  €9/hr

Industry:    On Request

Contact:

Admin: research.facilities@dcu.ie

Technical: stephen.fuller@dcu.ie


Fluorescence Spectrometer

Perkin Elmer LS55 Fluorescence Spectrometer

The Perkin Elmer LS 55 luminescence spectrometer is a very versatile instrument that allows measurement of fluorescence, phosphorescence, and chemiluminescence or bioluminescence of a sample. 

The Model LS 55 Series uses a pulsed Xenon lamp as a source of excitation. The sample is excited from 200 to 800 nm and the emission spectra recorded from 200 to 900 nm. Excitation and emission monochromators can be independently or synchronously scanned, while the Prescan mode is ideal for method development and locating of peak excitation and emission maxima.

Measurement Modes for the LS 55

  • Fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurement modes
  • Excitation, emission, constant wavelength synchronous, and constant energy synchronous spectral scanning
  • 3D excitation/emission scans, 3D synchronous and kinetic scans
  • Single and multiple wavelength kinetics
  • Simultaneous kinetics for multiple samples
  • Simple quantification by curve fitting with a number of fit algorithms

Prices:

Academic:  €5/hr

Industry :     On Request

Contact: 

Admin: research.facilities@dcu.ie

Technical: stephen.fuller@dcu.ie

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UV-VIS Scanning Spectrophotometry

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Shimadzu / UV-2600

Shimadzu UV-2600 Spectrometer. Used to facilitate the qualitative and quantitative analysis of compounds which absorb light in the UV-VIS region. This single monochromator, variable slit, double-beam instrument has a standard scanning range of 185-900nm but with the availableShimadzu ISR 2600 Plus Integrating Sphere it can extend the operating range into the near-IR region with an upper limit of 1,400nm. 

All instrument control & data acquisition is performed through the latest version of the Shimadzu UVProbe software, which includes built-in Validation Software as standard to support GLP/GMP requirements. Highly customisable acquisition Modules - SpectrumPhotometricand Kinetics - also facilitate a wide range of applications. 
 

  • Wavelength accuracy of ±0.3nm throughout the entire range
  • Wavelength repeatability of ±0.05nm
  • Scan rate range between 0.5 - 4,000 nm/min
  • Resolution of ±0.1nm
  • Photometric range between -5 to +5 Abs
  • Photometric accuracy as low as ±0.002 Abs
  • Photometric repeatability as low as ±0.001 Abs
  • Noise levels as low as 0.00003 Abs RMS (@ 500nm)
 

Shimadzu / UV-1800

Shimadzu UV-1800 Spectrometer. Used to facilitate the qualitative and quantitative analysis of compounds which absorb light in the UV-VIS region. This single monochromator, fixed slit, double-beam instrument has a standard scanning range of 190-1,100nm. 

Instrument control & data acquisition can be performed either in stand-alone mode directly via the display panel on the front of the instrument (using a USB flash drive to transfer analysis data) or through the latest version of the Shimadzu UVProbe software, which includes built-in Validation Software as standard to support GLP/GMP requirements. Highly customisable acquisition Modules (Spectrum, Photometric and Kinetics) also facilitate a wide range of applications.

  • Wavelength accuracy of ±0.3nm throughout the entire range
  • Wavelength repeatability of ±0.1nm
  • Resolution of ±1nm
  • Photometric range between -4 to +4 Abs
  • Photometric accuracy as low as ±0.002 Abs
  • Photometric repeatability as low as ±0.001Abs
  • Noise levels as low as 0.00005 Abs RMS (@ 700nm)

Prices:

Academic:  €5/hr

Industry:     On Request

(maximum usage - 200hrs /annum for this fee)

Contact:

Admin: research.facilities@dcu.ie

Technical: stephen.fuller@dcu.ie


FTIR Spectrometer

Perkin Elmer Spectrum Two

The Spectrum Two is suitable for a wide range of applications and includes a high performance detector, a standard sample compartment with sample slide mount and the comprehensive Spectrum 10™, and Spectrum 10™ Upgrade software interfaces. The system has a source range of 350 - 8,300 cm-1 and a diamond universal ATR accessory.

Prices:

Academic: €5/hr

Industry: On Request

Contact: 

Admin: research.facilities@dcu.ie

Technical: leah.nolan@dcu.ie

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Thermo Scientific Nicolet 6700 FTIR

  • Source range:  20 – 9,600 cm-1;
  • Beamsplitter range: 350 – 7,800 cm-1;
  • Detector range: 350 – 12,500 cm-1.
  • Includes an ATR accessory.

Prices:

Academic:  €5/hr

Industry:     On Request

Contact:

Admin: research.facilities@dcu.ie

Technical: leah.nolan@dcu.ie